在药物研发过程中，对总胆汁酸TBA的准确监控必不可少，那么，如何检测小鼠总胆汁酸呢？
老款试剂盒80470已被淘汰，建议使用新款的80471，更少的样本需求，更稳定的试剂保存，更稳定的实验结果。艾美捷 Crystal Chem高分文章引用的升级款小鼠总胆汁酸检测试剂盒，能很好的解决这一问题。该试剂盒检测原理如下：
小鼠总胆汁酸检测试剂盒是利用3-a羟基类固醇脱氢酶(3-aHSD)特有的酶学性质，以酶学技术为基础开发的。在NAD存在下，胆汁酸转化为3-酮类固醇和NADH。生成的NADH与硝基四唑蓝(NBT)反应形成染料。通过在540nm处测量吸光度来监测染料的形成，吸光度与小鼠样品中的胆汁酸浓度成正比。
Nature引用的CCM-80471超敏小鼠总胆汁酸检测试剂盒，You, Sangmin, et al. "Dysregulation of bile acids increases the risk for preterm birth in pregnant women." Nature communications 11.1 (2020): 1-15.
其它发表文章（仅摘取部分展示）：
1、Cao, Lijuan, et al. "Repression of intestinal transporters and FXR-FGF15 signaling explains bile acids dysregulation in experimental colitis-associated colon cancer."Oncotarget38 (2017): 63665.
2、Martinot, E., Baptissart, M., Véga, A. et al. Bile acid homeostasis controls CAR signaling pathways in mouse testis through FXRalpha. Sci Rep 7, 42182 (2017).
3、Lai, Qiuwen, et al. "E2F1 inhibits circulating cholesterol clearance by regulating Pcsk9 expression in the liver." JCI insight 2.10 (2017).
4、Zhang, Jun, et al. "Chronic over-expression of fibroblast growth factor 21 increases bile acid biosynthesis by opposing FGF15/19 action." EBioMedicine 15 (2017): 173-183.
5、Boué, Stephen, et al. "A novel gastrointestinal microbiome modulator from soy pods reduces absorption of dietary fat in mice." Obesity 24.1 (2016): 87-95.
6、Chin, Siew Hung, et al. "Opposing effects of reduced kidney mass on liver and skeletal muscle insulin sensitivity in obese mice." Diabetes 64.4 (2015): 1131-1141.