广义上的DNA甲基化是指DNA序列上特定的碱基在DNA甲基转移酶(DNA methyltransferase，DNMT)的催化作用下，以s一腺苷甲硫氨酸(S-adenosyl methionine，SAM)作为甲基供体，通过共价键结合的方式获得一个甲基基团的化学修饰过程。这种DNA甲基化修饰可以发生在胞嘧啶的C一5位、腺嘌呤的N一6位及鸟嘌呤的N一7位等位点。
许多研究发现，DNA甲基化模式的改变导致了的发生。在很多中都发现DNMTs的表达异常。这说明DNMTs在生物体内发挥着重要的作用。
DNMTs与的关联
艾美捷科技作为专业的生命科学领域解决方案供应商，为您推荐畅销的DNMT1, DNMT3A, DNMT3B定量试剂盒：
*每个试剂盒均被大量文献引用，见页面底部
DNMT酶活性分析试剂盒原理【DNMT1为例】：
EpiQuik的DNA甲基转移酶1检测试剂盒专为定量检测组织或细胞中的总Dnmt1而设计。在检测实验中，微孔上包被有本公司特有的Dnmt吸附基质。上样后，样品中的Dnmt1结合到基质上。被结合的Dnmt1能被特异性的Dnmt1抗体识别，可以通过类酶联免疫吸附反应后测定其比色度来定量，Dnmt1的量与显色程度成线性关系。
DNMT酶活性分析试剂盒优势：
1、操作十分快捷，3小时内可完成
2、安全的比色度检测方法，不接触放射性物质，无需进行抽提和色谱分析
3、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析
4、操作简便、结果可靠、统一的分析条件
5、适用于人与小鼠样品
《DNMT1试剂盒已发表文章》
Swathy B et. al. (January 2018). Understanding the influence of antipsychotic drugs on global methylation events and its relevance in treatment response. Epigenomics.
Li YC et. al. (September 2017). Procaine is a specific DNA methylation inhibitor with anti-tumor effect for human gastric cancer.J Cell Biochem.
Rajpathak SN et. al. (February 2017). Micro RNAs and DNA methylation are regulatory players in human cells with altered X chromosome to autosome balance. Sci Rep. 7:43235.
Cechinel LR et. al. (October 2016). Treadmill exercise induces age and protocol-dependent epigenetic changes in prefrontal cortex of Wistar rats. Behav Brain Res. 313:82-7.
Wu F et. al. (March 2016). Oral administration of Schisandra chinensis extract suppresses Dnmt1 expression in Kunming mice ovaries Genes Genom. :1-8.
Mytych J et. al. (February 2016). Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells. Mol Neurobiol.
Mukhopadhyay P et. al. (October 2015). Cigarette smoke induces proteasomal-mediated degradation of DNA methyltransferases and methyl CpG-/CpG domain-binding proteins in embryonic orofacial cells. Reprod Toxicol.
Yang G et. al. (July 2015). DNA methyltransferase 3, a target of microRNA-29c, contributes to neuronal proliferation by regulating the expression of brain-derived neurotrophic factor. Mol Med Rep. 12(1):1435-42.
Selokar NL et. al. (April 2015). Downregulation of DNA Methyltransferase 1 in Zona-Free Cloned Buffalo (Bubalus bubalis) Embryos by Small Interefering RNA Improves In Vitro Development But Does Not Alter DNA Methylation Level. Cell Reprogram.17(2):89-94.
Das DS et. al. (December 2014). Anti-Myeloma Activity of a Novel Free Radical Inducer Rrx-001 Blood. 124(21):4712-4712.
Chu M et. al. (April 2014). Hypermethylation-mediated transcriptional repression of TMPRSS2 in androgen receptor-negative prostate cancer cells. Exp Biol Med (Maywood).
Bielak-Zmijewska A et. al. (February 2014). A comparison of replicative senescence and doxorubicin-induced premature senescence of vascular smooth muscle cells isolated from human aorta. Biogerontology. 15(1):47-64.
Nettersheim D et. al. (December 2013). Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development. PLoS One. 8(12):e82881.
Wu Z et. al. (September 2013). Ultraviolet B enhances DNA hypomethylation of CD4+ T cells in systemic lupus erythematosus via inhibiting DNMT1 catalytic activity. J Dermatol Sci. 71(3):167-73.
Jang JY et. al. (February 2013). ANT2 suppression by shRNA may be able to exert anticancer effects in HCC further by restoring SOCS1 expression. Int J Oncol. 42(2):574-82.
Zhao J et. al. (January 2013). Genome-wide identification of Epstein-Barr virus-driven promoter methylation profiles of human genes in gastric cancer cells. Cancer. 119(2):304-12.
Desplats P et. al. (March 2011). Alpha-synuclein sequesters Dnmt1 from the nucleus: a novel mechanism for epigenetic alterations in Lewy body diseases. J Biol Chem. 286(11):9031-7.
Jang JY et. al. (September 2010). Short-hairpin RNA-induced suppression of adenine nucleotide translocase-2 in breast cancer cells restores their susceptibility to TRAIL-induced apoptosis by activating JNK and modulating TRAIL receptor expression.Mol Cancer. 9:262.
Hervouet E et. al. (June 2010). Disruption of Dnmt1/PCNA/UHRF1 interactions promotes tumorigenesis from human and mice glial cells. PLoS One. 5(6):e11333.
Liu Z et. al. (May 2009). Modulation of DNA methylation by a sesquiterpene lactone parthenolide. J Pharmacol Exp Ther.329(2):505-14.
Majid S et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4):662-70.
Roll JD et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol Cancer. 7:15.
00001. D. Roll et. al.(January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines Molecular Cancer.7(15) Full PDF Article
《DNMT3A试剂盒已发表文章》
Swathy B et. al. (January 2018). Understanding the influence of antipsychotic drugs on global methylation events and its relevance in treatment response. Epigenomics.
Coco M et. al. (November 2017). Effects of age and sex on epigenetic modification induced by an acute physical exercise.Medicine (Baltimore). 96(44):e8325.
Parira T et. al. (September 2017). Novel detection of post-translational modifications in human monocyte-derived dendritic cells after chronic alcohol exposure: Role of inflammation regulator H4K12ac. Sci Rep. 7(1):11236.
Mytych J et. al. (February 2016). Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells. Mol Neurobiol.
Horsburgh S et. al. (December 2015). Exercise-conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells. Physiol Rep. 3(12) Majid S et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4):662-70.
Roll JD et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol Cancer. 7:15.
《DNMT3B试剂盒已发表文章》
Coco M et. al. (November 2017). Effects of age and sex on epigenetic modification induced by an acute physical exercise.Medicine (Baltimore). 96(44):e8325.
Cechinel LR et. al. (October 2016). Treadmill exercise induces age and protocol-dependent epigenetic changes in prefrontal cortex of Wistar rats. Behav Brain Res. 313:82-7.
Mytych J et. al. (February 2016). Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells. Mol Neurobiol.
Horsburgh S et. al. (December 2015). Exercise-conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells. Physiol Rep. 3(12)
Zhao J et. al. (January 2013). Genome-wide identification of Epstein-Barr virus-driven promoter methylation profiles of human genes in gastric cancer cells. Cancer. 119(2):304-12.
Jin Y et. al. (June 2011). Homocysteine levels impact directly on epigenetic reprogramming in astrocytes. Neurochem Int.58(7):833-8.
Majid S et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4):662-70.
Roll JD et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol Cancer. 7:15.
艾美捷科技与国内外优秀的生物试剂供应商保持着密切的合作关系，目前已成为众多的中国总代理或一级代理，主要包括：Abbkine、Merck Millipore、Origene、AAT Bioquest、Cayman Chemical、Abnova 、Biovision、ProSpec、Hycult Biotech 、Equitech-Bio、Trevigen、Alomone Labs、Epigentek、ImmunoReagents 、Jackson、SouthernBiotech、US Biological 、Caisson Labs等，可以在时间为用户提供的专业资讯、完备的产品及物流服务。